From: Detection of subtle neurological alterations by the Catwalk XT gait analysis system
Version | CatWalk 7 | CatWalk XT |
---|---|---|
Settings | I. Detection settings involve multiple settings with no quantitative feedback of the outcome. | I. Detection settings involve fewer settings with quantitative feedback of the outcome. CatWalk XT even offers a function that searches for the most optimal settings automatically. |
II. Recording of video is based on defining start and stop zones, which can be swapped depending on the direction of movement of the animal in the walkway. | II. Recording of video is performed automatically. | |
III. Track files are given unique descriptions based on animal ID, run ID, and time. | III. User can define a trial list that includes animal ID, experiment group, and time point. These independent variables can later be used for data selection. | |
Acquisition | I. Footprints are captured at a speed of 50 Hz (PAL) to 60 Hz (NTSC). | I. Footprints are captured at a speed of 100Hz high with a high speed color camera. |
II. Acquisition must take place in complete darkness. | II. Acquisition can take place under red ambient light conditions. | |
III. Runs are captured if a user-definable maximum duration has not been reached. | III. Runs are labeled “compliant” if the run complies with user-definable duration interval and speed variation. All runs are captured, but for analysis, one can choose to only select “compliant” runs. | |
PAL: Phase Alternating Line | IV. Feedback is provided during acquisition when a user-definable number of “compliant” runs have been captured. | |
NTSC: National Television System Committee | ||
Analysis | I. Footprints are manually classified based on a black image where only footprints are visible. | I. With Automatic Footprint Classification, footprints are automatically classified. Other body parts that contact the glass plate are highlighted, so that these can easily be manually labeled or discarded. |
II. CatWalk provides a list of parameters, for a detailed assessment of the locomotion of your animal. | II. Video export for presentation purposes. | |
III. CatWalk XT provides data on three levels of abstraction: | ||
➢ raw data on video frame-by-frame basis | ||
➢ run statistics (equivalent to software 7.1) | ||
➢ trial statistics which provide the average for multiple runs that belong to the same trial (per animal, per time point) as if it was one combined run. | ||
Camera | Lens: 8.5 mm, Cosmicar, Japan Camera: TM-62EX, Pulnix, USA | Lens: 8.5 mm, Fujinon, China Camera: GP-3360, Gevicam, USA |